The Proteas will then have further group stage matches on 13 October against a qualifier, 16 October against England in Gqeberha, 19 October against Bangladesh in Durban and 22 October against Sri Lanka in Johannesburg.
The Wanderers will host the final on 21 November, with Newlands allocated the opening match of the group stages on 7 October, that will be between defending champions Australia and India.
Co-hosts Zimbabwe will mark their return to the 50-over World Cup after a 12-year absence when they take on a qualifying team at Queen's Sports Club in Bulawayo on 9 October.
Namibia will host the preliminary round of the competition, starting on 2 October, to determine who gets into the two groups.
The iconic game between arch-rivals India and Pakistan will take place at the Wanderers on 10 October, after the two Asian teams were placed in the same group.
The top three teams of each group will move into the Super 7 phase, with the better fourth-place finisher across the two groups taking the seventh spot.
The semi-finals will take place in Cape Town and Centurion on 17 and 18 November respectively.
Trevor Manuel, independent chairperson of the World Cup 2027 LOC Board, said: "South Africa, Zimbabwe and Namibia are ready to welcome the ICC Men's Cricket World Cup and supporters from around the world back to our continent.
"Southern Africa has a proud tradition of hosting major global sporting events, and we approach 2027 with the same confidence, ambition and determination to deliver a tournament worthy of the occasion.
"While the cricket will rightly take centre stage, this World Cup is also an opportunity to showcase the people, places and spirit that make Southern Africa so remarkable.
"We hope supporters will arrive in their multitudes for the cricket and leave with lasting memories of the warmth, energy, diversity and extraordinary welcome of our region."
World Cup groups:
Group A - Australia, India, Pakistan Afghanistan, co-hosts Zimbabwe and a qualifier
Group B - South Africa, New Zealand, England, Bangladesh, Sri Lanka and a qualifier
Australian stars Cameron Green and Matt Renshaw appeared to have added their names to the growing injury list during the third and final ODI against the Proteas on Wednesday night.
Australia avoided a 3-0 ODI series defeat as rain brought South Africa’s chase to an early end in Potchefstroom on Wednesday.
Proteas spinner Keshav Maharaj has jumped up to third in the latest ICC ODI bowling rankings after impressing in the first two games of the home series against Australia.
Aiden Markram says it was "nice having a little bit of time off" but is ready to contribute as the Proteas eye an ODI series victory against Australia at the Wanderers on Sunday.
